茶树GGPS基因家族的克隆、生物信息学和表达分析

牻牛儿基牻牛儿基焦磷酸合酶(Geranylgeranyl diphosphate synthase,GGPS)是萜类合成途径的结构酶,对植物生长发育具有重要意义。本研究通过RACE和RT-PCR方法克隆得到5条潜在的茶树GGPS序列,分别命名为CsGGPS1-4和CsGGPS9,其中CsGGPS9存在3条等位基因,分别是CsGGPS9-1、CsGGPS9-2和CsGGPS9-3,在系统进化树上与其他基因分成两支。蛋白质序列分析表明,茶树GGPS家族成员都具有polyprenyl_synt结构域,不存在信号肽序列。亚细胞定位预测结果显示,CsGGPS1、CsGGPS2和CsGGPS4定位在叶绿体上,CsGGPS3和CsGGPS9定位在线粒体上。通过Swiss Model进行三维建模,结合"three-floor"模型对茶树GGPS家族成员的功能进行预测,预测结果显示,CsGGPS1、CsGGPS2和CsGGPS4是GGPS;CsGGPS3是异源二聚体形式的牻牛儿基焦磷酸合酶的小亚基;CsGGPS9的催化主产物是碳链数大于30的异戊烯基焦磷酸。q RT-PCR分析表明,CsGGPS1整体表达丰度较低,仅在一芽二叶中表达量稍高;CsGGPS2在茶树各个组织中均有表达,在花中表达量最高,且花发育过程中表达量先上升后下降;CsGGPS3在叶和幼根中的表达量高于花,花发育过程中表达平稳;CsGGPS4在茶树各个组织中表达量数值相近,在花发育过程中表达量变化趋势与CsGGPS2相同;CsGGPS9的表达量在成熟叶中显著低于幼嫩叶片。     

A stochastic surplus production model in continuous time

Surplus production modelling has a long history as a method for managing data‐limited fish stocks. Recent advancements have cast surplus production models as state‐space models that separate random variability of stock dynamics from error in observed indices of biomass. We present a stochastic surplus production model in continuous time (SPiCT), which in addition to stock dynamics also models the dynamics of the fisheries. This enables error in the catch process to be reflected in the uncertainty of estimated model parameters and management quantities. Benefits of the continuous‐time state‐space model formulation include the ability to provide estimates of exploitable biomass and fishing mortality at any point in time from data sampled at arbitrary and possibly irregular intervals. We show in a simulation that the ability to analyse subannual data can increase the effective sample size and improve estimation of reference points relative to discrete‐time analysis of aggregated annual data. Finally, subannual data from five North Sea stocks are analysed with particular focus on using residual analysis to diagnose model insufficiencies and identify necessary model extensions such as robust estimation and incorporation of seasonality. We argue that including all known sources of uncertainty, propagation of that uncertainty to reference points and checking of model assumptions using residuals are critical prerequisites to rigorous fish stock management based on surplus production models.     

Drivers of Setaria pumila (Poir.) Roem. et Schult growth and impact on forage quality in lowland Switzerland meadows

Invasive grasses (especially Setaria pumila (Poir.) Roem. et Schult) increasingly threaten meadows and pastures as a consequence of human impact and climate change. We conducted a study in 2012 and in 2013 to better understand the growing cycle and the influence of S. pumila on forage quality of lowland meadows. We observed a rapid increase of S. pumila presence and phytomass on the southern side of the Alps across the growing season. We measured (i) above‐ground phytomass, with a modified Corral‐Fenlon method; (ii) botanical composition using the linear point quadrat method; and (iii) grassland chemical composition by NIRs (near‐infrared spectroscopy) analyses. To test the hypothesis that S. pumila summer growth is related to specific climatic conditions, meteorological data (temperature, precipitation and evapotranspiration) were collected from meteorological stations near the study sites. Total phytomass was sorted into S. pumila and other species. We used a generalized linear mixed models (GLMM) and found the abundance of S. pumila to be inversely correlated with rainfall and the presence of other species, but positively correlated with temperature increase. The increase of S. pumila above‐ground phytomass production was linked to a reduction of forage quality.     

夜间灯光数据DMSP/OLS在贫困地区亮化度模型构建中的应用

贫困县亮化度一定程度上反映着贫困县居民的生活水平发展状况,但与贫困区经济收入等相关指标的定量关系不太明确。针对该情况,提出了一种应用灯光数据的亮度统计数据,结合与贫困县经济收入等密切相关的经济规模指标、人口数量、财政收入等来定量研究贫困县亮化度模型,并选取样本贫困县对该模型进行验证。经数据验证,该模型具有较高的可信度。     

基于ARIMA模型的我国大豆单产预测分析

在我国大豆单产光合潜力和"农业生态区划"(AEZ)潜力基础上,运用ARIMA(自回归单整移动平均)模型预测了2020年前我国大豆单产。结果表明:我国大豆单产最大潜力为3 400 kg·hm~(-2),而2017、2018、2019和2020年单产将分别为1 899,1 926,1 954和1 982 kg·hm~(-2),分别是最大潜力的55.85%、56.65%、57.47%和58.29%。这意味着:未来提高我国大豆单产尚有较大空间,应保持高产耕地生产力与改良中低产田土并重。研究结果旨在为我国大豆生产提供决策参考信息。     

Finding suitable growth models for turbot (Scophthalmus maximus L.) in aquaculture 1 (length application)

Growth data of two different commercial turbot (Scophthalmus maximus) strains reared in recirculating aquaculture systems were analysed with the aim to determine the most suitable model for turbot. To assess the model performance three different criteria were used: (1) The mean percentage deviation between the estimated length and actual length; (2) the residual standard error with corresponding degrees of freedom and (3) the Akaike information criterion. The analyses were carried out for each strain separately, for sexes within strains and for a pooled data set containing both strains and sexes. We tested a pre‐selection of six models, containing three to four parameters. Models were of monomolecular shape or sigmoid shape with a flexible point of inflection including the special case of monomolecular shape in defined cases of their parameters. The 4‐parametric Schnute model achieved best fit in 62% of all cases and criteria tested, followed by the also 4‐parametric generalized Michaelis–Menten equation in 48% and the 4‐parametric Janoschek model (38%). The von Bertalanffy growth function achieved only 29%, Brody 24% and a new flexible function 19% best fit. In a 1–1000 day growth‐simulation sigmoid shaped curves were produced by the Schnute model in 71% of cases. The Janoschek and the Michaelis–Menten model each produced sigmoid curves in 57% of all cases. This indicates that a flexible 4‐parametric function reflects the growth curve of turbot the best and that this curve is rather sigmoid than monomolecular shaped.     

扬州市稻田高效种养模式应用现状及发展对策

近年来,扬州市根据国家农业供给侧结构性调整要求,充分利用自然资源禀赋,开展了多种形式稻田种养模式的示范与推广。本文阐述了扬州市稻鸭共作、稻虾共作以及稻田改作荷藕-虾共作等高效种养模式的应用现状,以单纯种稻为对照,比较了各种模式的生产效益;分析了稻田高效种养模式示范推广过程中存在的主要问题,并提出了促进稻田高效种养模式可持续发展的若干对策。     

福建省文化产业发展影响因素及对策研究

以1999-2013年福建省文化产业发展数据为样本,利用多元回归模型对影响福建省文化产业发展的主要因素进行实证分析.结论显示,经济规模、文化产业从业人员数量以及文化产业财政支出这3个要素对福建省文化产业的发展有重要影响.针对影响因素,提出大力发展国民经济,为文化产业发展提供保障;转变政府职能,推进文化产业市场化;培养文化产业人才,提升文化产业内涵;注重消费者诉求,开拓需求市场等对策来提升福建省文化产业.     

感应热处理工艺对拖拉机轴类零件扭转强度的影响

对驱动轮工艺轴进行了7种工艺方案的感应热处理表面强化,然后对工艺轴进行扭转破坏试验。通过对驱动轮工艺轴扭转断裂的研究,为驱动轮轴及承受扭力的轴类零件结构设计、工艺流程设计和热处理技术要求提供指导,从而提高其扭转疲劳强度和使用寿命。     

北京市公园绿地投资标准测算

公园绿地作为"唯一有生命力"的城市基础设施,一直以来其建设和投资标准不像道路、管线等基础睡设施那般明确,而随着近年来城市发展对生态环境的日益重视,各类公园绿地项目逐渐增多。为了加强公园绿地项目在前期设计和审批阶段的科学性、合理性,文章结合北京市当前园林绿化的实际情况,按照"一亩地公园"模型的思路,根据不同的投资工程,建立了投资标准测算模型,测算了投资指标,尝试为公园绿地的投资标准确定提供一种新的思路。